Agarose gel for RNA and DNA

1g agarose in 100mL of

Microwave solution in a flask for 0h 3m 0s until the agarose is dissolved. Microwave for 0h 1m 0s thereafter 0h 0m 30s

Allow to cool before adding 1µL in the gel medium before completely cool. Then pour into the gel mold

Make sure that your plate is balanced and the teeth are in. Decide if you need a long ladder or a short weather.

Add 5µL in the gel medium within the chromatography chamber

250-300ng C1.V1 = C2.V2

V1 = (10)(50)/RNA conc. (from nanodrop)

Add the V1 sample of RNA to 1:1 ratio of to each sample

Heat up the ladder and RNA samples to 70°C for 0h 5m 0s for the gel

is already in the samples for the PCR; then use the

Add 5µL of ladder and samples to gel wells

For RNA: 80V for 1h 0m 0s

For DNA: 80V for 0h 40m 0s

*Note: can check gel after 0h 10m 0s to see if there is product showing on the gel