Adolf & Du et al., 2024

Frank Adolf

Published: 2024-02-07 DOI: 10.17504/protocols.io.e6nvwdrozlmk/v1

ASAPCRN

proteasome

core particle

20S proteasome

chaperone

molecular machine

multiprotein complex

POMP

PAC1

PAC2

PAC3

PAC4

propeptide

protease

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Abstract

These protocols details methods for cloning, expression, purification and structural determination by transmission electron cryo-microscopy of 20S CPs and assembly intermediates.

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