A rapid screening platform to coculture bacteria within tumor spheroids

Tetsuhiro Harimoto, Dhruba Deb, Tal Danino

Published: 2022-07-28 DOI: 10.1038/s41596-022-00723-5

Tumor spheroids

Coculture

Bacterial screening

Microbial therapies

High-throughput platform

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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The prevalence of tumor-colonizing bacteria along with advances in synthetic biology are leading to a new generation of living microbial cancer therapies. Because many bacterial systems can be engineered to recombinantly produce therapeutics within tumors
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

Sepich-Poore, G. D. et al. The microbiome and human cancer. Science 371, eabc4552 (2021).
Nejman, D. et al. The human tumor microbiome is composed of tumor type-specific intracellular bacteria. Science 368, 973–980 (2020).
Zhou, S., Gravekamp, C., Bermudes, D. & Liu, K. Tumour-targeting bacteria engineered to fight cancer. Nat. Rev. Cancer 18, 727–743 (2018).
Harimoto, T. & Danino, T. Engineering bacteria for cancer therapy. Emerg. Top. Life Sci. 3, 623–629 (2019).
Chien, T., Doshi, A. & Danino, T. Advances in bacterial cancer therapies using synthetic biology. Curr. Opin. Syst. Biol. 5, 1–8 (2017).
Bullman, S. et al. Analysis of Fusobacterium persistence and antibiotic response in colorectal cancer. Science 358, 1443–1448 (2017).
Uemura, N. et al. Helicobacter pylori infection and the development of gastric cancer. N. Engl. J. Med. 345, 784–789 (2001).
Kalaora, S. et al. Identification of bacteria-derived HLA-bound peptides in melanoma. Nature 592, 138–143 (2021).
Geller, L. T. et al. Potential role of intratumor bacteria in mediating tumor resistance to the chemotherapeutic drug gemcitabine. Science 357, 1156–1160 (2017).
Pleguezuelos-Manzano, C. et al. Mutational signature in colorectal cancer caused by genotoxic pks+ E. coli. Nature 580, 269–273 (2020).
Lehouritis, P., Springer, C. & Tangney, M. Bacterial-directed enzyme prodrug therapy. J. Control. Release 170, 120–131 (2013).
Isabella, V. M. et al. Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria. Nat. Biotechnol. 36, 857–864 (2018).
Flickinger, J. C. Jr., Rodeck, U. & Snook, A. E. Listeria monocytogenes as a vector for cancer immunotherapy: current understanding and progress. Vaccines (Basel) 6, 48 (2018).
Toso, J. F. et al. Phase I study of the intravenous administration of attenuated Salmonella typhimurium to patients with metastatic melanoma. J. Clin. Oncol. 20, 142–152 (2002).
Roberts, N. J. et al. Intratumoral injection of Clostridium novyi-NT spores induces antitumor responses. Sci. Transl. Med. 6, 249ra111 (2014).
Nemunaitis, J. et al. Pilot trial of genetically modified, attenuated Salmonella expressing the E. coli cytosine deaminase gene in refractory cancer patients. Cancer Gene Ther. 10, 737–744 (2003).
Helmink, B. A., Khan, M. A. W., Hermann, A., Gopalakrishnan, V. & Wargo, J. A. The microbiome, cancer, and cancer therapy. Nat. Med. 25, 377–388 (2019).
Riglar, D. T. & Silver, P. A. Engineering bacteria for diagnostic and therapeutic applications. Nat. Rev. Microbiol. 16, 214–225 (2018).
Forbes, N. S. Engineering the perfect (bacterial) cancer therapy. Nat. Rev. Cancer 10, 785–794 (2010).
Harimoto, T. et al. Rapid screening of engineered microbial therapies in a 3D multicellular model. Proc. Natl Acad. Sci. USA 116, 9002–9007 (2019).
Chien, T. et al. Enhancing the tropism of bacteria via genetically programmed biosensors. Nat. Biomed. Eng. 6, 94–104 (2022).
Zuniga, A. et al. Engineered L-lactate responding promoter system operating in glucose-rich and anoxic environments. ACS Synth. Biol. 10, 3527–3536 (2021).
Kasper, S. H. et al. Colorectal cancer-associated anaerobic bacteria proliferate in tumor spheroids and alter the microenvironment. Sci. Rep. 10, 5321 (2020).
Yano, S. et al. Tumor-targeting Salmonella typhimurium A1-R decoys quiescent cancer cells to cycle as visualized by FUCCI imaging and become sensitive to chemotherapy. Cell Cycle 13, 3958–3963 (2014).
Gao, S. et al. Development of oxytolerant Salmonella typhimurium using radiation mutation technology (RMT) for cancer therapy. Sci. Rep. 10, 3764 (2020).
Sadaghian Sadabad, M. et al. A simple coculture system shows mutualism between anaerobic faecalibacteria and epithelial Caco-2 cells. Sci. Rep. 5, 17906 (2015).
Zheng, D. W. et al. Optically-controlled bacterial metabolite for cancer therapy. Nat. Commun. 9, 1680 (2018).
Wang, S. B. et al. Bacteria-assisted selective photothermal therapy for precise tumor inhibition. Adv. Funct. Mater. 29, 1904093 (2019).
Wang, X. N., Niu, M. T., Fan, J. X., Chen, Q. W. & Zhang, X. Z. Photoelectric bacteria enhance the in situ production of tetrodotoxin for antitumor therapy. Nano Lett. 21, 4270–4279 (2021).
Song, J. et al. A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting. Sci. Rep. 8, 6394 (2018).
Hong, J. W., Song, S. & Shin, J. H. A novel microfluidic co-culture system for investigation of bacterial cancer targeting. Lab Chip 13, 3033–3040 (2013).
Din, M. O. et al. Synchronized cycles of bacterial lysis for in vivo delivery. Nature 536, 81–85 (2016).
Zhang, W., Mao, S., He, Z., Wu, Z. & Lin, J. M. In situ monitoring of fluid shear stress enhanced adherence of bacteria to cancer cells on microfluidic chip. Anal. Chem. 91, 5973–5979 (2019).
Mokrani, N. et al. Magnetotactic bacteria penetration into multicellular tumor spheroids for targeted therapy. Annu. Int. Conf. IEEE Eng. Med. Biol. Soc. 2010, 4371–4374 (2010).
Suh, S. et al. Nanoscale bacteria-enabled autonomous drug delivery system (NanoBEADS) enhances intratumoral transport of nanomedicine. Adv. Sci. (Weinh.) 6, 1801309 (2019).
Kasinskas, R. W. & Forbes, N. S. Salmonella typhimurium specifically chemotax and proliferate in heterogeneous tumor tissue in vitro. Biotechnol. Bioeng. 94, 710–721 (2006).
Deng, Y., Liu, S. Y., Chua, S. L. & Khoo, B. L. The effects of biofilms on tumor progression in a 3D cancer-biofilm microfluidic model. Biosens. Bioelectron. 180, 113113 (2021).
Brackett, E. L., Swofford, C. A. & Forbes, N. S. Microfluidic device to quantify the behavior of therapeutic bacteria in three-dimensional tumor tissue. Methods Mol. Biol. 1409, 35–48 (2016).
Goers, L., Freemont, P. & Polizzi, K. M. Co-culture systems and technologies: taking synthetic biology to the next level. J. R. Soc. Interface 11, 20140065 (2014).
Swofford, C. A., St Jean, A. T., Panteli, J. T., Brentzel, Z. J. & Forbes, N. S. Identification of Staphylococcus aureus α-hemolysin as a protein drug that is secreted by anticancer bacteria and rapidly kills cancer cells. Biotechnol. Bioeng. 111, 1233–1245 (2014).
Quail, D. F. & Joyce, J. A. Microenvironmental regulation of tumor progression and metastasis. Nat. Med. 19, 1423–1437 (2013).
Yu, B. et al. Explicit hypoxia targeting with tumor suppression by creating an “obligate” anaerobic Salmonella Typhimurium strain. Sci. Rep. 2, 436 (2012).
Ryan, R. M. et al. Bacterial delivery of a novel cytolysin to hypoxic areas of solid tumors. Gene Ther. 16, 329–339 (2009).
Friedrich, J., Seidel, C., Ebner, R. & Kunz-Schughart, L. A. Spheroid-based drug screen: considerations and practical approach. Nat. Protoc. 4, 309–324 (2009).
Fong, E. L., Harrington, D. A., Farach-Carson, M. C. & Yu, H. Heralding a new paradigm in 3D tumor modeling. Biomaterials 108, 197–213 (2016).
Osswald, A. et al. Three-dimensional tumor spheroids for in vitro analysis of bacteria as gene delivery vectors in tumor therapy. Microb. Cell Fact. 14, 199 (2015).
Bhave, M. S., Hassanbhai, A. M., Anand, P., Luo, K. Q. & Teoh, S. H. Effect of heat-inactivated Clostridium sporogenes and its conditioned media on 3-dimensional colorectal cancer cell models. Sci. Rep. 5, 15681 (2015).
Co, J. Y., Margalef-Catala, M., Monack, D. M. & Amieva, M. R. Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases. Nat. Protoc. 16, 5171–5192 (2021).
Zhang, J. et al. Coculture of primary human colon monolayer with human gut bacteria. Nat. Protoc. 16, 3874–3900 (2021).
Aguilar, C. et al. Organoids as host models for infection biology—a review of methods. Exp. Mol. Med. 53, 1471–1482 (2021).
Danino, T., Lo, J., Prindle, A., Hasty, J. & Bhatia, S. N. In vivo gene expression dynamics of tumor-targeted bacteria. ACS Synth. Biol. 1, 465–470 (2012).
Canale, F. P. et al. Metabolic modulation of tumours with engineered bacteria for immunotherapy. Nature 598, 662–666 (2021).
Doi, Y., Wachino, J. I. & Arakawa, Y. Aminoglycoside resistance: the emergence of acquired 16S ribosomal RNA methyltransferases. Infect. Dis. Clin. North Am. 30, 523–537 (2016).
Codini, M. et al. Gentamicin arrests cancer cell growth: the intriguing involvement of nuclear sphingomyelin metabolism. Int. J. Mol. Sci. 16, 2307–2319 (2015).
Lee, C. H., Wu, C. L. & Shiau, A. L. Endostatin gene therapy delivered by Salmonella choleraesuis in murine tumor models. J. Gene Med. 6, 1382–1393 (2004).
Yu, B. et al. Obligate anaerobic Salmonella typhimurium strain YB1 treatment on xenograft tumor in immunocompetent mouse model. Oncol. Lett. 10, 1069–1074 (2015).
Danino, T. et al. Programmable probiotics for detection of cancer in urine. Sci. Transl. Med. 7, 289ra284 (2015).

A rapid screening platform to coculture bacteria within tumor spheroids

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