6M GITC
Jacquelina.Woods
Published: 2021-12-02 DOI: 10.17504/protocols.io.bybbpsin
Abstract
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method provides a reagent formula required in the extraction of RNA from viral concentrates using the RNeasy and Zymo kits.
Steps
1.
Aseptically mix 7.09g 4.5mL
2.
Bring total volume up to 10mLwith additional sterile distilled or ultrapure water
3. Solution is only stable for 1 month after preparation.
Store at 2-8Room temperaturein the dark (or in light occluding tube)
Note
