4% Paraformaldehye in .1M PB preparation

Add 400mL of MilliQ water to a 1000mL

Equipment

Value	Label
Milli-Q® Integral Water Purification System for Ultrapure Water	NAME
filter	TYPE
Millipore Sigma	BRAND
ZRXQ010WW	SKU

Add stir bar in beaker and set on hot plate at 50°C

Add 5 drops (500ul) of 10N NaOH (from 60ml dropper)

(this makes the solution basic to help dissolve the paraformaldehyde)

Weigh 20g of Paraformaldehyde and add to MilliQ water.

(Stir about 1h 0m 0s or until all parafromaldehyde is dissolved and solution is clear)

Make 1Molarity (M) Phosphate buffer at PH 7.4

Prepare 1Molarity (M) Soduim Phosphate Dibasic Dihydrate (Store stock at Room temperature .)

Prepare 1Molarity (M) Sodium Phospate Monobasic Anyhydrous (store stock at Room temperature )

To make 100mL add 19mL plus 81mL (store as stock at Room temperature )

Add 50mL to paraformaldehyde solution.

Test PH and adjust solution to final desired pH using HCl or NaOH.

Top off to 500ml with MilliQ water.

Filter solution through .2um CA member filter.

Lable container with '4% paraformaldehyed in .1M PB' and date. Store at 4°C

(Can also be aliqouted into

and stored at -20°C for 3 months.)