10x Multiome Sample Processing

Allen Institute for Brain Science

Published: 2024-05-17 DOI: 10.17504/protocols.io.bp2l61mqrvqe/v2

Abstract

Protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries, and for rapid generation of 3' transcriptomic-NGS-ready-single-cell-libraries from the same pool of transposed nuclei.

"Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Numbers 1U01MH114812-01 and 1U19MH114830-01. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health".

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