0.3M Sodium Cacodylate Buffer pH 7.4 Stock Solution Recipe
Mark Ellisman, Thomas Deerinck, Marianne Martone
Published: 2023-07-14 DOI: 10.17504/protocols.io.q26g74x79gwz/v1
Abstract
Standard preparation protocol for stock 0.3M sodium cacodylate buffer.
Steps
1.
Add 900 ml of DDH2O to 64.209 grams of sodium cacodylate in a large 1.0L or greater container, such as a capped glass bottle. Shake and wait until powder is fully dissolved.
2.
Maximum HCL concentration is 12.18mole/liter (36-38%). Start with 1.3mL of 12.18 M HCL per 900 ml of sodium cacodylate dissolved solution. Make sure solution is mixed thoroughly.
3.
Check pH. Keep adding 50-100μl of HCL, mix, and check pH again until pH 7.4 has been reached. (Approximately 1.4 ml of conc. HCL total).
4.
Top off to 1000ml with DDH2O.
5.
Do a final pH check and keep stock solution refrigerated at 4C.
