Tutorial: guidelines for annotating single-cell transcriptomic maps using automated and manual methods

Tallulah S. Andrews, Zoe A. Clarke, Jawairia Atif, Delaram Pouyabahar, Brendan T. Innes, Sonya A. MacParland, Gary D. Bader

Published: 2021-05-23 DOI: 10.1038/s41596-021-00534-0

single-cell transcriptomics

workflow

cell annotation

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software tools

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Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Single-cell transcriptomics can profile thousands of cells in a single experiment and identify novel cell types, states and dynamics in a wide variety of tissues and organisms. Standard experimental protocols and analysis workflows have been developed to
Main
Step 1: automatic cell annotation
Step 2: expert manual cell annotation
- Annotating cell states and gradients
Step 3: annotation verification
Experimental design considerations
- The impact of experimental and analysis parameters on annotation
- Workflow recommendations
Concluding remarks
- References
- References
References
ACKNOWLEDGMENTS

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Tutorial: guidelines for annotating single-cell transcriptomic maps using automated and manual methods

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