Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genotyping

Isabel Weisheit, Joseph A. Kroeger, Rainer Malik, Benedikt Wefers, Peter Lichtner, Wolfgang Wurst, Martin Dichgans, Dominik Paquet

Published: 2021-02-16 DOI: 10.1038/s41596-020-00481-2

CRISPR-induced OnTEs

qgPCR

SNP genotyping

genome editing

allele detection

AI 解读

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The recent CRISPR revolution has provided researchers with powerful tools to perform genome editing in a variety of organisms. However, recent reports indicate widespread occurrence of unintended CRISPR-induced on-target effects (OnTEs) at the edited site
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

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Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genotyping

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