Rolling circle amplification (RCA)-based DNA hydrogel

Rui Zhang, Jianpu Tang, Dayong Yang, Chi Yao

Published: 2021-10-28 DOI: 10.1038/s41596-021-00621-2

DNA hydrogel

Rolling circle amplification

3D hydrogel network

Bone marrow mesenchymal stem cells

Cell delivery

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

查看全部

Sections

Figures

References

DNA hydrogels have unique properties, including sequence programmability, precise molecular recognition, stimuli-responsiveness, biocompatibility and biodegradability, that have enabled their use in diverse applications ranging from material science to bi
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

Yuk, H., Lu, B. & Zhao, X. Hydrogel bioelectronics. Chem. Soc. Rev. 48, 1642–1667 (2019).
Zhang, Y. S. & Khademhosseini, A. Advances in engineering hydrogels. Science 356, eaaf3627 (2017).
Bao, M., Xie, J. & Huck, W. T. S. Recent advances in engineering the stem cell microniche in 3D. Adv. Sci. 5, 1800448 (2018).
Chaudhuri, O. et al. Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nat. Mater. 15, 326–334 (2016).
Kraehenbuehl, T. P., Langer, R. & Ferreira, L. S. Three-dimensional biomaterials for the study of human pluripotent stem cells. Nat. Methods 8, 731–736 (2011).
Baker, B. M. et al. Cell-mediated fibre recruitment drives extracellular matrix mechanosensing in engineered fibrillar microenvironments. Nat. Mater. 14, 1262–1268 (2015).
Culver, H. R., Clegg, J. R. & Peppas, N. A. Analyte-responsive hydrogels: Intelligent materials for biosensing and drug delivery. Acc. Chem. Res. 50, 170–178 (2017).
Seeman, N. C. Nucleic acid junctions and lattices. J. Theor. Biol. 99, 237–247 (1982).
Yang, D. et al. DNA materials: bridging nanotechnology and biotechnology. Acc. Chem. Res. 47, 1902–1911 (2014).
Roh, Y. H., Ruiz, R. C., Peng, S., Lee, J. B. & Luo, D. Engineering DNA-based functional materials. Chem. Soc. Rev. 40, 5730–5744 (2011).
Wang, D., Hu, Y., Liu, P. & Luo, D. Bioresponsive DNA hydrogels: beyond the conventional stimuli responsiveness. Acc. Chem. Res. 50, 733–739 (2017).
Shao, Y., Jia, H., Cao, T. & Liu, D. Supramolecular hydrogels based on DNA self-assembly. Acc. Chem. Res. 50, 659–668 (2017).
Kahn, J. S., Hu, Y. & Willner, I. Stimuli-responsive DNA-based hydrogels: from basic principles to applications. Acc. Chem. Res. 50, 680–690 (2017).
Li, J. et al. Self-assembly of DNA nanohydrogels with controllable size and stimuli-responsive property for targeted gene regulation therapy. J. Am. Chem. Soc. 137, 1412–1415 (2015).
Wang, J. et al. Clamped hybridization chain reactions for the self-assembly of patterned DNA hydrogels. Angew. Chem. Int. Ed. 56, 2171–2175 (2017).
Nöll, T. et al. Construction of three-dimensional DNA hydrogels from linear building blocks. Angew. Chem. Int. Ed. 53, 8328–8332 (2014).
Li, J. et al. Functional nucleic acid-based hydrogels for bioanalytical and biomedical applications. Chem. Soc. Rev. 45, 1410–1431 (2016).
English, M. A. et al. Programmable crispr-responsive smart materials. Science 365, 780–785 (2019).
Li, F., Tang, J., Geng, J., Luo, D. & Yang, D. Polymeric DNA hydrogel: Design, synthesis and applications. Prog. Polym. Sci. 98, 101163 (2019).
Um, S. H. et al. Enzyme-catalysed assembly of DNA hydrogel. Nat. Mater. 5, 797–801 (2006).
Cheng, E. et al. A pH-triggered, fast-responding DNA hydrogel. Angew. Chem. Int. Ed. 48, 7660–7663 (2009).
Lee, J. B. et al. A mechanical metamaterial made from a DNA hydrogel. Nat. Nanotechnol. 7, 816–820 (2012).
Yao, C. et al. Double rolling circle amplification generates physically cross-linked DNA network for stem cell fishing. J. Am. Chem. Soc. 142, 3422–3429 (2020).
Tang, J. et al. Super-soft and super-elastic DNA robot with magnetically-driven navigational locomotion for cell delivery in confined space. Angew. Chem. Int. Ed. 59, 2490–2495 (2020).
Geng, J. et al. A fluorescent biofunctional DNA hydrogel prepared by enzymatic polymerization. Adv. Healthc. Mater. 7, 1700998 (2018).
Merindol, R., Delechiave, G., Heinen, L., Catalani, L. H. & Walther, A. Modular design of programmable mechanofluorescent DNA hydrogels. Nat. Commun. 10, 528 (2019).
Hamada, S. et al. Dynamic DNA material with emergent locomotion behavior powered by artificial metabolism. Sci. Robot. 4, eaaw3512 (2019).
Madl, C. M., Heilshorn, S. C. & Blau, H. M. Bioengineering strategies to accelerate stem cell therapeutics. Nature 557, 335–342 (2018).
De Luca, M. et al. Advances in stem cell research and therapeutic development. Nat. Cell Biol. 21, 801–811 (2019).
Qian, X. et al. Untethered recyclable tubular actuators with versatile locomotion for soft continuum robots. Adv. Mater., e1801103 (2018).
Wang, C. et al. Soft ultrathin electronics innervated adaptive fully soft robots. Adv. Mater. 30, e1706695 (2018).
Cangialosi, A. et al. DNA sequence-directed shape change of photopatterned hydrogels via high-degree swelling. Science 357, 1126–1130 (2017).
Yang, C. & Suo, Z. Hydrogel ionotronics. Nat. Rev. Mater. 3, 125–142 (2018).
Gao, T. et al. Design and fabrication of flexible DNA polymer cocoons to encapsulate live cells. Nat. Commun. 10, 2946 (2019).
Ye, D. et al. Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction. Nat. Protoc. 15, 2163–2185 (2020).
Ellington, A. D. & Szostak, J. W. In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818–822 (1990).
Li, L. et al. Nucleic acid aptamers for molecular diagnostics and therapeutics: Advances and perspectives. Angew. Chem. Int. Ed. Engl. 60, 2221–2231 (2021).
McCarthy, S. A., Davies, G. L. & Gun’ko, Y. K. Preparation of multifunctional nanoparticles and their assemblies. Nat. Protoc. 7, 1677–1693 (2012).

Rolling circle amplification (RCA)-based DNA hydrogel

Supplementary information

推荐阅读