Open Field Behavior with head-mount

One-month post-surgery, mice were habituated to head-mounted equipment over 2 days in their home cages.

On day 1, an actual or mock wireless inertial sensor (~2.5 cm H x 1 cm L x 0.5 cm W with ~ 2.5-3.0 cm antennae, ~1.8 g weight) glued to the 4-position connector (Harwin Inc., M52-040023V0445) was attached to the implanted receptacle connector on the skull cap.

Individual mice roamed freely in the home cage for 1 hour.

On day 2, an actual wireless inertial sensor and mono fiberoptic patchcord (200/220 μm diameter, 0.22 NA; Doric DFP_200/220/900-0.22_2m_DF0.7-2FC) was attached to the skullcap via a mating sleeve. Patchcords were attached to 1x2 fiber-optic rotary joint (intensity division, 0.22 NA; Doric,

FRJ_1x2i_FC-2FC) and mice roam freelyin home cage for 1 hour.

Sensor/patchcord habituated mice were anesthetized by isoflurane, attached to equipment, subjected to calibration protocol (see guidelines)

Individual mice were placed in an open field box inside a sound insulated chamber and allowed to behave freely inside the box for 75 minutes.

The open field box is made of 410 x 400 mm grey opaque acrylicwalls and a 410 x 400 mm white matte acrylic base.

The wireless inertial sensor (~1.8 g in weight, WEAR wireless sensor v1.1; Champalimaud Scientific Hardware Platform) conveys motion information sampled at 200 hz (set on WEAR v1.3.2 software; Champalimaud Scientific Hardware Platform) to a receiver base-station (Harp basestation v1.1 or v. 1.2, Assembly v0, Harp v1.4, Firmware v1.5; Champalimaud Scientific Hardware Platform), which conveys the information to the experimental computer running a Bonsai script(Bonsai⁴⁵ editor v2.3.1,RRID:SCR_017218) to capture and record motion data and video information. Video was captured with a camera(Flea3 FL3-U3-I3Y3M(17450451), Point Grey Research)coupled to a 1/2” format lens (NMV-6WA, Navitar).