Nano-CUT&Tag for multimodal chromatin profiling at single-cell resolution

José Ramón Bárcenas-Walls, Federico Ansaloni, Bastien Hervé, Emilia Strandback, Tomas Nyman, Gonçalo Castelo-Branco, Marek Bartošovič

Published: 2023-12-21 DOI: 10.1038/s41596-023-00932-6

single-cell resolution

chromatin profiling

Nano-CUT&Tag

nanobody-Tn5 fusion

epigenetic modalities

AI 解读

Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

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Combining surface-accessible Ag and Au colloidal nanomaterials with SERS for in situ analysis of molecule–metal interactions in complex solution environments

Generation of mouse–human chimeric embryos

Labeling and tracking of immune cells in ex vivo human skin

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing

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Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
Acknowledgements
Extended Data Fig. 1 Workflows for activity assessment of in-house-produced Tn5.
Extended Data Fig. 2 Nano-CT library structure.

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Nano-CUT&Tag for multimodal chromatin profiling at single-cell resolution

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