Multiplexed single-cell analysis of organoid signaling networks

Jahangir Sufi, Xiao Qin, Ferran Cardoso Rodriguez, Yong Jia Bu, Petra Vlckova, María Ramos Zapatero, Mark Nitz, Christopher J. Tape

Published: 2021-09-07 DOI: 10.1038/s41596-021-00603-4

organoids

single-cell analysis

multiplexed mass cytometry

PTM signaling

CyGNAL

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–6, Supplementary Tables 1 and 2 and Supplementary Method.

Reporting Summary

Supplementary Table 3

Debarcoding key for 35-plex TOBis

Supplementary Table 4

Debarcoding key for 126-plex TOBis

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Multiplexed single-cell analysis of organoid signaling networks

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