Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids

Hans Clevers, Johanna F. Dekkers, Esmée J. van Vliet, Norman Sachs, Jennifer M. Rosenbluth, Oded Kopper, Heggert G. Rebel, Ellen J. Wehrens, Carol Piani, Jane E. Visvader, Carla S. Verissimo, Sylvia F. Boj, Joan S. Brugge, Anne C. Rios

Published: 2021-03-09 DOI: 10.1038/s41596-020-00474-1

xenotransplantation

breast cancer organoids

long-term culture

genetic manipulation

orthotopic transplantation

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Organoid technology has revolutionized the study of human organ development, disease and therapy response tailored to the individual. Although detailed protocols are available for the generation and long-term propagation of human organoids from various or
Introduction
Materials
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Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

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Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids

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