Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution

Jörg Enderlein, Arindam Ghosh, Alexey I. Chizhik, Narain Karedla

Published: 2021-06-06 DOI: 10.1038/s41596-021-00558-6

Graphene-induced energy transfer

Metal-induced energy transfer

Single-molecule imaging

Nanoscopy

Axial resolution

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Supplementary information

Supplementary Information

Supplementary Notes 1–4 and Supplementary Fig. 1.

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Supplementary information

Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006).
Rust, M. J., Bates, M. & Zhuang, X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3, 793–796 (2006).
Hess, S. T., Girirajan, T. P. & Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91, 4258–4272 (2006).
Heilemann, M. et al. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew. Chem. Int. Ed. 47, 6172–6176 (2008).
Sharonov, A. & Hochstrasser, R. M. Wide-field subdiffraction imaging by accumulated binding of diffusing probes. Proc. Natl Acad. Sci. USA 103, 18911–18916 (2006).
Schnitzbauer, Joerg et al. Super-resolution microscopy with DNA-PAINT. Nat. Protoc. 12.6, 1198 (2017).
Balzarotti, F. et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355, 606–612 (2016).
Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett. 19, 780–782 (1994).
Gwosch, K. C. et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat. Methods 17, 217–224 (2020).
Shtengel, G. et al. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).
Aquino, D. et al. Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores. Nat. Methods 8, 353–359 (2011).
Schmidt, R. et al. Spherical nanosized focal spot unravels the interior of cells. Nat. Methods 5, 539–544 (2008).
Hell, S. W., Schmidt, R. & Egner, A. Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses. Nat. Photonics 3, 381–387 (2009).
Juette, M. F. et al. Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nat. Methods 5, 527–529 (2008).
Huang, B., Wang, W., Bates, M. & Zhuang, X. Three-dimensional superresolution imaging by stochastic optical reconstruction microscopy. Science 319, 810–813 (2008).
Backlund, M. P. et al. Simultaneous, accurate measurement of the 3D position and orientation of single molecules. Proc. Natl Acad. Sci. USA 109, 19087–19092 (2012).
Santos, D. et al. Topography of cells revealed by variable-angle total internal reflection fluorescence microscopy. Biophys. J. 111, 1316–1327 (2016).
Loerke, D., Stühmer, W. & Oheim, M. Quantifying axial secretory-granule motion with variable-angle evanescent-field excitation. J. Neurosci. Methods 119, 65–73 (2002).
Winterflood, C. M. et al. Nanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy. Phys. Rev. Lett. 105, 108103 (2010).
Deschamps, J., Mund, M. & Ries, J. 3D superresolution microscopy by supercritical angle detection. Opt. Express, 22, 29081–29091 (2014).
Chizhik, A. I., Rother, J., Gregor, I., Janshoff, A. & Enderlein, J. Metal-induced energy transfer for live cell nanoscopy. Nat. Photonics 8, 124–127 (2014).
Karedla, N. et al. Single-molecule metal-induced energy transfer (smMIET): resolving nanometer distances at the single-molecule level. ChemPhysChem 15, 705–711 (2014).
Ghosh, A. et al. Graphene-based metal-induced energy transfer for sub-nanometre optical localization. Nat. Photonics 13, 860–865 (2019).
Roy, R., Hohng, S. & Ha, T. A practical guide to single-molecule FRET. Nat. Methods 5, 507–516 (2008).
Baronsky, T. et al. Cell–substrate dynamics of the epithelial-to-mesenchymal transition. Nano Lett. 17, 3320–3326 (2017).
Chance, R., Prock, A. & Silbey, R. Molecular fluorescence and energy transfer near interfaces. Adv. Chem. Phys. 37, 1–65 (1978).
Enderlein, J. Single-molecule fluorescence near a metal layer. Chem. Phys. 247, 1–9 (1999).
Gregor, I. et al. Metal-induced energy transfer. Nanophotonics 8, 1689–1699 (2019).
Chizhik, A. I. & Enderlein, J. Metal-induced energy transfer imaging. in Nanoscale Photonic Imaging (eds Salditt, T. et al.) 227–239 (Springer, 2020).
Simoncelli, S., Makarova, M., Wardley, W. & Owen, D. M. Toward an axial nanoscale ruler for fluorescence microscopy. ACS Nano 11, 11762–11767 (2017).
Chizhik, A. M. et al. Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy. Mol. Biol. Cell 29, 846–851 (2018).
Chizhik, A. M. et al. Three-dimensional reconstruction of nuclear envelope architecture using dual-color metal-induced energy transfer imaging. ACS Nano 11, 11839–11846 (2017).
Zelená, A. et al. Time-resolved MIET measurements of blood platelet spreading and adhesion. Nanoscale 12, 21306–21315 (2020).
Isbaner, S. et al. Axial colocalization of single molecules with nanometer accuracy using metal-induced energy transfer. Nano Lett. 18, 2616–2622 (2018).
Karedla, N. et al. Three-dimensional single-molecule localization with nanometer accuracy using metal-induced energy transfer (MIET) imaging. J. Chem. Phys. 148, 204201 (2018).
Moerland, R. J. & Hoogenboom, J. P. Subnanometer-accuracy optical distance ruler based on fluorescence quenching by transparent conductors. Optica 3, 112–117 (2016).
Wei, D. et al. Ultrathin rechargeable all-solid-state batteries based on monolayer graphene. J. Mater. Chem. A 1, 3177–3181 (2013).
Chizhik, A. I., Gregor, I., Ernst, B. & Enderlein, J. Nanocavity-based determination of absolute values of photoluminescence quantum yields. ChemPhysChem 14, 505–513 (2013).
Schneider, F., Ruhlandt, D., Gregor, I., Enderlein, J. & Chizhik, A. I. Quantum yield measurements of fluorophores in lipid bilayers using a plasmonic nanocavity. J. Phys. Chem. Lett. 8, 1472–1475 (2017).
Oleksiievets, N. et al. Wide-field fluorescence lifetime imaging of single molecules. J. Phys. Chem. A 124, 3494–3500 (2020).
Maruyama, Y. & Charbon, E. An all-digital, time-gated 128X128 spad array for on-chip, filter-less fluorescence detection. 16th International Solid-State Sensors, Actuators and Microsystems Conference, 1180-1183. (2011)
Böhmer, M. & Enderlein, J. Orientation imaging of single molecules by wide-field epifluorescence microscopy. J. Opt. Soc. Am. B 20, 554–559 (2003).
Patra, D., Gregor, I. & Enderlein, J. Image analysis of defocused single molecule images for three-dimensional molecule orientation studies. J. Phys. Chem. A 108, 6836 (2004).
Backer, A. S. & Moerner, W. E. Determining the rotational mobility of a single molecule from a single image: a numerical study. Opt. Express 23, 4255–4276 (2015).
Isbaner, S. et al. Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging. Opt. Express 24, 9429–9445 (2016).
GWDG Project Management Service. https://projects.gwdg.de/projects/miet/repository/raw/MIET_GUI.zip?rev=ZIP
Richter, R. P., Bérat, R. & Brisson, A. R. Formation of solid-supported lipid bilayers: an integrated view. Langmuir 22, 3497–3505 (2006).
Meleard, P., Bagatolli, L. A. & Pott, T. Giant unilamellar vesicle electroformation: from lipid mixtures to native membranes under physiological conditions. Methods Enzymol. 465, 161–176 (2009).

Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution

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