Disposal of Environmental Samples from Water Bodies Infested with Zebra Mussels

Malcolm A Barnard, Stephen Powers

Published: 2023-02-10 DOI: 10.17504/protocols.io.eq2lynoymvx9/v2

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Abstract

Zebra Mussels ( Dreissena polymorpha ) are a highly invasive species that have been invading water bodies around the United States and beyond. This protocol for sample disposal for sample collected from know zebra mussel infested water bodies to prevent spread though local water treatment facilities by bleaching the samples to kill any larvae or other potential zebra mussels in the sample water.

Steps

Sample Killing

1.

Pour all environmental sample water from bottles into a dish pan.

2.

Save the bottles for acid washing

Safety information
Do not add bleach to bottles being cleaned using an acid bath and acid as the combination of acid and bleach produces chlorine gas.
.

3.

Add 50 mL of bleach per 1 L of sample water.

4.

Let the bleached sample sit for a minimum of four hours if not until the next day.

Sample Disposal

5.

Dispose of bleached sample down drain with excess water or other waste pathway as described by local environmental health and safety guidelines.

6.

Save bin for future sample disposal or bleach work.

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