Construction and use of an adaptive optics two-photon microscope with direct wavefront sensing

Pantong Yao, Rui Liu, Thomas Broggini, Martin Thunemann, David Kleinfeld

Published: 2023-10-31 DOI: 10.1038/s41596-023-00893-w

two-photon microscopy

adaptive optics

wavefront sensing

neocortex imaging

guidestar

浏览 1163

Supplementary information

Supplementary Data 1

Assembly drawings.

Supplementary Data 2

Part drawings.

Supplementary Data 3

ZEMAX file.

Supplementary Table 1

Comprehensive parts list including current prices and vendor links.

Supplementary Code 1

MATLAB scripts.

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

In vitro reconstitution of COPII vesicles fromArabidopsis thalianasuspension-cultured cells

Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins

Monomicellar assembly to synthesize structured and functional mesoporous carbonaceous nanomaterials

An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells

Measuring DNA modifications with the comet assay: a compendium of protocols

Design and build of small-scale magnetic soft-bodied robots with multimodal locomotion

A practical guide to scanning light-field microscopy with digital adaptive optics

Reproducible mass spectrometry data processing and compound annotation in MZmine 3

Genetic in vivo engineering of human T lymphocytes in mouse models

Digital postprocessing and image segmentation for objective analysis of colorimetric reactions

查看全部

Sections

Figures

References

Supplementary information

Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73–76 (1990).
Kleinfeld, D., Mitra, P. P., Helmchen, F. & Denk, W. Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. Proc. Natl Acad. Sci. USA 95, 15741–15746 (1998).
Svoboda, K., Denk, W., Kleinfeld, D. & Tank, D. W. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385, 161–165 (1997).
Dong, C. et al. Fluorescence imaging of neural activity, neurochemical dynamics, and drug-specific receptor conformation with genetically encoded sensors. Annu. Rev. Neurosci. 45, 273–294 (2022).
Chen, T. W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295–300 (2013).
Marvin, J. S. et al. Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nat. Methods 15, 936–939 (2018).
Aggarwal, A. et al. Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission. Nat. Methods 20, 925–935 (2023).
Villette, V. et al. Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice. Cell 179, 1590–1608 e23 (2019).
Kleinfeld, D. et al. Can one concurrently record electrical spikes from every neuron in a mammalian brain? Neuron 103, 1005–1015 (2019).
Jun, J. J. et al. Fully integrated silicon probes for high-density recording of neural activity. Nature 551, 232–236 (2017).
Ohki, K., Chung, S., Ch’ng, Y. H., Kara, P. & Reid, R. C. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433, 597–603 (2005).
Xu, S. et al. Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles. Science 370, eabb2494 (2020).
Hampson, K. M. et al. Adaptive optics for high-resolution imaging. Nat. Rev. Methods Prim. 1, 68 (2021).
Wang, K. et al. Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue. Nat. Commun. 6, 7276 (2015).
Wang, K. et al. Rapid adaptive optical recovery of optimal resolution over large volumes. Nat. Methods 11, 625–628 (2014).
Liu, R., Li, Z., Marvin, J. S. & Kleinfeld, D. Direct wavefront sensing enables functional imaging of infragranular axons and spines. Nat. Methods 16, 615–618 (2019).
Debarre, D. et al. Image-based adaptive optics for two-photon microscopy. Opt. Lett. 34, 2495–2497 (2009).
Tang, J., Germain, R. N. & Cui, M. Superpenetration optical microscopy by iterative multiphoton adaptive compensation technique. Proc. Natl Acad. Sci. USA 109, 8434–8439 (2012).
Ji, N., Milkie, D. E. & Betzig, E. Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues. Nat. Methods 7, 141–147 (2010).
Streich, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat. Methods 18, 1253–1258 (2021).
Rodriguez, C. et al. An adaptive optics module for deep tissue multiphoton imaging in vivo. Nat. Methods 18, 1259–1264 (2021).
Aviles-Espinosa, R. et al. Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy. Biomed. Opt. Express 2, 3135–3149 (2011).
Yardeni, T., Eckhaus, M., Morris, H. D., Huizing, M. & Hoogstraten-Miller, S. Retro-orbital injections in mice. Lab Anim. (NY) 40, 155–160 (2011).
Drew, P. J. et al. Chronic optical access through a polished and reinforced thinned skull. Nat. Methods 7, 981–984 (2010).
Holtmaat, A. et al. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat. Protoc. 4, 1128–1144 (2009).
Ji, N., Sato, T. R. & Betzig, E. Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex. Proc. Natl Acad. Sci. USA 109, 22–27 (2012).
Ji, N. Adaptive optical fluorescence microscopy. Nat. Methods 14, 374–380 (2017).
Wang, C. et al. Multiplexed aberration measurement for deep tissue imaging in vivo. Nat. Methods 11, 1037–1040 (2014).
Qin, Z. et al. Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo. Light Sci. Appl. 9, 79 (2020).
Meng, G. et al. High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo. eLife 8, e40805 (2019).
Resendez, S. L. et al. Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses. Nat. Protoc. 11, 566–597 (2016).
Qin, Z. et al. Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes. Sci. Adv. 6, eabc6521 (2020).
Noll, R. J. Zernike polynomials and atmospheric-turbulence. J. Opt. Soc. Am. 66, 207–211 (1976).
Berlage, C. et al. Deep tissue scattering compensation with three-photon F-SHARP. Optica 8, 1613–1619 (2021).
Papadopoulos, I. N., Jouhanneau, J.-S., Poulet, J. F. A. & Judkewitz, B. Scattering compensation by focus scanning holographic aberration probing (F-SHARP). Nat. Photonics 11, 116–123 (2016).
Podgorski, K. & Ranganathan, G. Brain heating induced by near-infrared lasers during multiphoton microscopy. J. Neurophysiol. 116, 1012–1023 (2016).
Prevedel, R. et al. Fast volumetric calcium imaging across multiple cortical layers using sculpted light. Nat. Methods 13, 1021–1028 (2016).
Shih, A. Y. et al. Two-photon microscopy as a tool to study blood flow and neurovascular coupling in the rodent brain. J. Cereb. Blood Flow. Metab. 32, 1277–1309 (2012).
Yang, G., Pan, F., Parkhurst, C. N., Grutzendler, J. & Gan, W. B. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nat. Protoc. 5, 201–208 (2010).

关键词

two-photon microscopy

adaptive optics

wavefront sensing

neocortex imaging

guidestar

扫码咨询