Confirming circRNA expression by qPCR.pdf Xianjun Dong Published: 2023-06-23 DOI: 10.17504/protocols.io.e6nvwd6nzlmk/v1 ASAPCRN AI 解读 浏览 1115 Abstract This protocol delineates a qPCR method to confirm the expression of circRNAs extracted from brain samples. Attachments Confirming circRNA expression by qPCR.pdf Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols An MSstats workflow for detecting differentially abundant proteins in large-scale data-independent acquisition mass spectrometry experiments with FragPipe processing High-throughput cultivation and identification of bacteria from the plant root microbiota A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys CODEX multiplexed tissue imaging with DNA-conjugated antibodies Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids Raman spectral cytopathology for cancer diagnostic applications Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases The use of methanol as a C1 building block 查看全部 The Treadmill-Based Tripping Perturbation Fall Trip Stumble Perturbation Disturbance MSD Gold Streptavidin Antibody Preparation and Plate Run Protocol Buffer Exchange Biotinylate the Antibody Sulfo-tag the Antibody MSD ELISA protein quantification streptavidin antibody protein Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography ASAPCRN Protein Lysate Preparation Protocol (Culture) ASAPCRN Automation Protocol for High-Efficiency and High-Quality Genomic DNA Extraction from Saccharomyces Cerevisiae laboratory automation synthetic biology Saccharomyces Cerevisiae DNA extraction Dissociation of EBs using Worthington Kit ASAPCRN Barcode Composition by Overlap-Extension PCR FFPE Blocking and Sectioning - UMN TMCs URMC TriState SenNet Mouse Lung Digestion cellular senescence lung tissue Dissociation Spatial Transcriptomics for FFPE utilizing 10x Genomics Visium 查看全部 Taste-Associative Learning in Rats: Conditioned Immunosuppression with Cyclosporine A to Study the Neuro-Immune Network behaviorally conditioned immunosuppression conditioned taste avoidance cyclosporine A learned immune responses rats taste-immune associative learning Acute Myeloid Leukemia Minimal Residual Disease Detection: The Difference from Normal Approach acute myeloid leukemia AML flow cytometry minimal residual disease MRD How to Illuminate the Dark Proteome Using the Multi-omic OpenProt Resource alternative ORF alt-ORF OpenProt small ORF sORF Coated Latex Beads as Artificial Cells for Quantitative Investigations of Receptor/Ligand Interactions beads flow cytometry quantification stimulation Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans aging Caenorhabditis elegans epigenetics histone post-translational modifications histone variants mass spectrometry ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents humanized mouse model immune oncology patient-derived xenograft (PDX) models tumor-infiltrating lymphocytes CRISPR Genome Editing Made Easy Through the CHOPCHOP Website Cas9 CRISPR CRISPRi/a gene knock-out genome editing guide design knock-down knock-in nickase RNA targeting In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells human pluripotent stem cells (hPSCs) motor neurons neuromesodermal progenitors 查看全部
