Anti-condensation agent

Make a mixture of 0.05% Triton X-100 and 20% ethanol in a 50 mL test tube.

For 20 mL, mix:

4mL

0.1mL

16mL

Treating lids with the anti-condensation agent should be done at least a day before using the plates to study worm behaviour.

In a Class II Microbiological Safety Hood, pour ca. 3-4mL of the anti-condensation agent on the inside of the lids and leave for 0h 0m 30s.

Ensure all of the lid is covered by the anti-condensation agent.

Pour the liquid back into the 50 mL tube for later use, or onto new lids to be treated.

Leave lids to dry for ca 1h 0m 0s.

After drying, you will most likely see rings of anti-condensation agent residue on the plate. Wipe these off the lids with Kimberly-Clark Kimwipes until there are no signs of these rings.