U54 SCENT 10X Genomics Visium Protocol
Karen Abramson, Simon Gregory
Published: 2023-06-12 DOI: 10.17504/protocols.io.6qpvr3xozvmk/v1
Abstract
This document outlines the 10X Genomics Visium Protocol used for normal lung and colon tissues for SCENT at Duke University.
Steps
10X Genomics Visium Protocol
1.
Once slides received, the following 10x Genomics protocol is followed:
2.
10x Genomics kit is used – Visium (v1) Spatial for FFPE Gene Expression Kit, Human Transcriptome.
3.
Libraries sequenced on Illumina sequencers to a read depth of a least 25k reads/spot, with read lengths of 28x10x10x50.
