Tail Suspension Test
Ariadna Laguna, Miquel Vila, Núria Peñuelas, Marina Lorente Picón
Published: 2023-11-23 DOI: 10.17504/protocols.io.6qpvr3222vmk/v1
Abstract
Tail Suspension Test for mice
Steps
1.
Suspend animals by their tails with tape in a suspension bar.
2.
To avoid the tail climbing behavior, pass a 2cm methacrylate tube through the tail before suspending the animal.
3.
Quantify the escape-oriented behaviors (i.e. fore and hind limbs movement) during six minutes.
4.
Calculate the total immobilization time (s) as the sum of all the time the animal was not performing any escape-oriented behavior.
