TS Spurrs - primary fixation Karnovsky's - tissue (TM - 013)
sandra.crameri
Published: 2023-05-09 DOI: 10.17504/protocols.io.6qpvr4jypgmk/v1
Abstract
This method is used for conventional processing of tissue to Spurr's resin.
Steps
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1.
SAN:
SPEC No:
OPERATOR & STEPS:
OPERATOR & STEPS:
CONVENTIONAL
2.
2.5% volume 4% volume 0.1Molarity (M) for at least 0h 40m 0s
3.
Wash 0.1Molarity (M) Sorenson's Phosphate Buffer 07.2 (300mosmol/kg) for 0h 15m 0s
4.
1% volume 1h 0m 0s
5.
70% volume 0h 20m 0s
6.
95% volume 0h 20m 0s
7.
100% volume 0h 20m 0s
8.
100% volume 0h 20m 0s
9.
50% volume 50% volume 0h 30m 0s
10.
100% volume 0h 30m 0s
11.
100% volume 1h 0m 0s
12.
Oven polymerise overnight at 65°C
