S. elongatus stock revival
Akashdutta
Published: 2021-10-22 DOI: 10.17504/protocols.io.bzcxp2xn
Abstract
Stocks are more permanent methods of storing bacteria than plates. These stocks can be recovered by pouring them into small volumes of BG-11 and growing under low light levels. As the culture grows, it can be passaged into higher volumes and appropriate antibiotics can be added.
Steps
1. Do not add any antibiotics
Add your stock to 2mL BG-11 medium in a test tube and grow it overnight.
Note
2.
Transfer this to a 50mL flask and make up the culture to 10mL in volume.
3.
Once it reaches a lime green colour (#32CD32), add a fresh medium to make the culture up to 50mL culture in a 150mL
4.
Further, make this culture up to 100mL in a 250mL flask once it returns to lime green.
