RNA extraction and PCR protocol Enrico Bagnoli, Miratul Muqit Published: 2024-05-14 DOI: 10.17504/protocols.io.81wgbz7r3gpk/v1 ASAPCRN AI 解读 浏览 1103 Abstract This protocol details the extraction of RNA and PCR analysis. Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq Generation of heart-forming organoids from human pluripotent stem cells Design and fabrication of wearable electronic textiles using twisted fiber-based threads Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo Chromatin accessibility profiling by ATAC-seq Deoxyfluorination of phenols for chemoselective18F-labeling of peptides Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening 查看全部 Conditioned Media Concentration with Amicon Ultra Centrifugal Filters Conditioned Media Centrifugal Filters Proteomics Secretome SASP Collecting needle and branch samples for terpenoid and expression analysis mCherry-YIPF4 Immunoprecipitation V2 ASAPCRN Protocol for Safe, Affordable, and Reproducible Isolation of SARS-CoV-2 RNA from Wastewater RNA Wastewater Viral Concentration Variants RT-qPCR PEG TRIzol Wastewater-based epidemiology Nutrient solution for rice hydroponics culture rice hydroponics Yoshida's solution salinity Guidelines for microneutralization testing of human antibodies to West Nile virus Reproduction Analysis Short-Term Freezing of Anopheles stephensi (Mosquito) Larvae Combinatorial selective ER-phagy remodels the ER during neurogenesis ASAPCRN ER-phagy induced neurons axons whole cell proteomics differentiation live cell fluorescence microscopy Keima flux autophagy Wake Forest University Health Sciences Manual of Procedures Biospecimen Collection and Processing V4 查看全部 Generation of 3D Tumor Spheroids with Encapsulating Basement Membranes for Invasion Studies spheroid basement membrane invasion 3D-model Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High-Throughput Genotyping Protocol for Any Type of Mutation 3Rs replacement reduction refinement automation genotyping molecular biology mouse model PCR reproducibility Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host-Microbe Interactions fungal genomics maize plant genomics plant-microbe interactions unique molecular identifiers Ustilago maydis virulence factors ezTrack—A Step-by-Step Guide to Behavior Tracking behavioral analysis fear conditioning freeze position tracking Assessing Protein Synthesis and Degradation Rates in Arabidopsis thaliana Using Amino Acid Analysis 13CO2 labeling protein degradation protein synthesis Hepatitis E Virus: Isolation, Propagation, and Quantification HEV isolation propagation virus quantification Murine Norovirus: Additional Protocols for Basic and Antiviral Studies baculovirus expression circular polymerase extension reaction murine norovirus neonatal infections tuft cell-enriched murine intestinal enteroid culture Analysis of Genetic Ancestry from NGS Data Using EthSEQ ancestry analysis NGS SNPs Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles EVs exosomes large-scale preparation MSCs Genomic Epidemiology Analysis of Infectious Disease Outbreaks Using TransPhylo genomic epidemiology infectious disease outbreak phylogenetics transmission analysis 查看全部
