Protocol3_HEK293T cells and mRNA transfections.pdf angelica.gonzalez Published: 2024-01-27 DOI: 10.17504/protocols.io.36wgq3zyklk5/v1 AI 解读 浏览 1130 Abstract HEK293T cells and mRNA transfections Attachments Protocol3_HEK293T cells and mRNA transfections.pdf Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Fabrication of angstrom-scale two-dimensional channels for mass transport Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy Profiling native pulmonary basement membrane stiffness using atomic force microscopy Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes 查看全部 Conditioned Media Concentration with Amicon Ultra Centrifugal Filters Conditioned Media Centrifugal Filters Proteomics Secretome SASP Collecting needle and branch samples for terpenoid and expression analysis mCherry-YIPF4 Immunoprecipitation V2 ASAPCRN Protocol for Safe, Affordable, and Reproducible Isolation of SARS-CoV-2 RNA from Wastewater RNA Wastewater Viral Concentration Variants RT-qPCR PEG TRIzol Wastewater-based epidemiology Nutrient solution for rice hydroponics culture rice hydroponics Yoshida's solution salinity Guidelines for microneutralization testing of human antibodies to West Nile virus Reproduction Analysis Short-Term Freezing of Anopheles stephensi (Mosquito) Larvae Combinatorial selective ER-phagy remodels the ER during neurogenesis ASAPCRN ER-phagy induced neurons axons whole cell proteomics differentiation live cell fluorescence microscopy Keima flux autophagy Wake Forest University Health Sciences Manual of Procedures Biospecimen Collection and Processing V4 查看全部 Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome Generation of Bispecific Antibodies by Functionalized Poly-ADP-Ribose Polymers antibody bispecific antibody chemical conjugation immunotherapy PARP1 poly-ADP-ribose post-translational modification High-Throughput Gene Replacement in Aspergillus fumigatus Aspergillus fumigatus fusion PCR genome-wide knockout library high-throughput gene editing Human Umbilical Cord Mesenchymal Stem Cell–Based in vitro Model for Neurotoxicity Testing cytotoxicity test mesenchymal stem cells neuron-like cells neuronal markers Culture of Rat Primary Cortical Cells for Microelectrode Array (MEA) Recordings to Screen for Acute and Developmental Neurotoxicity acute subchronic and chronic exposure microelectrode array (MEA) recordings neuronal activity analysis neurotoxicity screening rat primary cortical cultures Hi-C 3.0: Improved Protocol for Genome-Wide Chromosome Conformation Capture chromatin interactions chromosome conformation capture cross-linking Hi-C restriction enzyme Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Chloroplast Transformation in Arabidopsis Arabidopsis biolistic transformation chloroplast transformation plastid transformation transplastomic plant 查看全部
