Preparing Agarose Gel

1X TAE Buffer* SYBRsafe

• Agarose powder
• Weigh boat
• 500mL Erlenmeyer flask
• Gel Electrophoresis tray
• Gel Electrophoresis comb
• Tape

Tape up the gel electrophoresis tray and add well comb

Add 200mL of 1X TAE buffer to the Erlenmeyer flask

Weigh the right amount of agarose powder - depends on the expected PCR products: smaller than 500 bp: 2% agarose gel – 4 gr/200mL; bigger than 500 bp: 1% agarose gel – 2 gr/200mL).

Microwave for 2-3 minutes

Carefully remove flask from microwave and add 20uL of SYBRsafe to the gel solution. Swirl to mix.

Slowly pour the mixture in to the electrophoresis tray

Let cool for at least 30 minutes (no longer than 2 hours)

Clean the Erlenmeyer flask to remove excess agarose gel