Picking worms
Bonnie Evans
Published: 2021-12-08 DOI: 10.17504/protocols.io.b2p5qdq6
Abstract
Transferring worms using a platinum wire pick
Steps
1.
Light the ethanol burner. Take care that there is nothing directly above it, eg. papers attached to shelf.
2.
Place the platinum wire part of the pick in the flame to sterilise it. Let it cool before touching the plate.
3.
Pick up some bacteria from the plate with the platinum wire.
4.
Look down the microscope to identify the worm you want to pick (e.g for L4 worms, look for a white semi-circle in the worm mid-section). Gently touch the worm with the bacteria to pick it up.
5.
Transfer the worm to the new plate by gently placing the pick on the agar and allowing it to crawl out of the bacteria on the wire. Be careful not to penetrate the agar.
