Open field test
mariangela.massarocenere
Published: 2024-01-31 DOI: 10.17504/protocols.io.5qpvo3677v4o/v1
Abstract
The open field test is a simple test to evaluate general motor and explorative behavior in rodents
Steps
1.
Habituate the animal for 1 h to the testing room before the test
2.
Place the animal in the center of open field cylindrical arena (100 cm diameter) with dark walls (35 cm) and floor, under dim lighting (300 lux)
3.
Leave the animal free to explore the arena for 10min and record with a video camera suspended above the arena
4.
Track the center and the periphery with video tracking software (EthoVision - Noldus, The Netherlands)
5.
Clean the apparatus every time between animals
