Modified Leeming and Notman Agar

Amy Gladfelter

Published: 2023-06-02 DOI: 10.17504/protocols.io.kxygxm3ywl8j/v1
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Abstract

Media recipe for growth of Malassezia species.

Before start

Attachments

Malassezia media_1.pdf

Steps

1.

bacteriological peptone (Oxoid) 10g

2.

yeast extract 2g

3.

desiccated ox bile (Oxoid) 8g

4.

glucose 10g

5.

glycerol monostearate 0.5g

6.

agar 15g

7.

glycerol, 50% 20mL

8.

tween 60 5mL

9.

olive oil 20mL

10.

dH2O 955mL

11.

Sterilize by autoclaving.

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