Longmire lysis buffer
Abigail Wells, Linda Park
Published: 2021-09-10 DOI: 10.17504/protocols.io.bx4xpqxn
Abstract
Lysis buffer recipe (Longmire et al 1997):
To make 1 liter
Steps
To make 1 L of Longmire
1.
975 ml double-distilled water
2.
100 ml of 1 M Tris-HCL, pH 8.0
3.
200 ml of 0.5 M EDTA, pH8.0
4.
2 ml of 5 M NaCl
5.
25 ml of 20% SDS (w/v) Filter the buffer with an autofill PES bottle top filtration device (sterile 500ml, 0.22 µm)
