JMN-MSMP Muscle Single Cell Isolation
ccherry
Published: 2023-09-27 DOI: 10.17504/protocols.io.j8nlko8xxv5r/v2
Abstract
Single cell isolation from muscle
Steps
Single cell isolation
1.
Tissue was finely diced in RPMI 1640 (Gibco) with 1.6 Wunsh U/mL Liberase TL (Roche Diagnostics) and 0.2 mgl/mL deoxyribonuclease I (Roche Diagnostics).
2.
Finely diced tissue was incubated at 37C for 45 minutes.
3.
Digested tissues were ground subsequently through 100um and 70um strainers (Thermo Fisher Scientific).
4.
Cells were centrifuged at 300g for 5 minutes and washed twice with 1x PBS
