Human metagenomics protocols Payami lab

This is a collection of protocols that details the entire process of conducting a human microbiome study from start to end. It includes (1) consent form, to enroll subjects with permission to store and share data (2) data collection protocols designed to be self-administered by subjects (Environmental & Family History Questionnaire, Gut Microbiome Questionnaire, instructions for saliva collection, and instructions for stool collection), (3) codes for bioinformatics and statistical data analysis, (4) workflow at a glance created according to STORM guidelines and (5) final product, the manuscript, which describes the whole process from start to end using metagenomics of Parkinson’s disease implicates the gut microbiome in multiple disease mechanisms.

1. Informed Consent Form used for enrolling subjects into study. This consent allowis long-term storage of samples and sharing data.pdf 2a. Data collection protcocol _Environmnetal u0026 Family History Questionnaire_Self adminsitered by subjects.pdf 2b. Data collection protcocol_Gut Microbiome Questionnaire_Self adminsitered by subjects (1).pdf 2c. Data collection protocol_Saliva Collection Instruction_Self adminsitered by subjects.pdf 2d. Data collection protcocol_Stool Collection Instruction _Self adminsitered by subjects.pdf 3a. Data analysis code_bioinformatics and statistics .pdf 3b. Data analysis code_bioinformatics and statistics.Rmd 4. Workflow at a glance_ STORM chart.pdf 5. Final product_describes the whole process from start to end_Nat Comm PMID 34408160 .pdf