Hematoxylin & Eosin Protocol For Leica ST5020 Automated Stainer

Angela Denn

Published: 2023-11-23 DOI: 10.17504/protocols.io.x54v9mozqg3e/v2

Abstract

This Hematoxylin and Eosin protocol is for the Leica ST5020 Automated multistainer. You can use it as a guide to developing your own protocol, catered to the needs of your researchers. This template can be modified for manual staining but best and most reproducible results are easier to achieve with an automated stainer.

Before start

Steps

H&E Stain

1.

Reagent layout for multi stainer use.

2.

Program for H&E protocol.

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