Genomic Signature Pattern Analysis of the HIV-1 Through Computational Approach Sushanta Kumar Barik Published: 2021-08-08 DOI: 10.17504/protocols.io.bw7sphne HIV-1 Genomic Signature Protease Reverse transcriptase AI 解读 浏览 1244 Abstract ). Steps1. 2. 3. 4. 5. 5 6. 6 7. 7 推荐阅读 Nature Protocols Protocols IO Current Protocols Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility Reproducible mass spectrometry data processing and compound annotation in MZmine 3 Production and use of antigen tetramers to study antigen-specific B cells Probing the free-state solution behavior of drugs and their tendencies to self-aggregate into nano-entities Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning Design of metal-mediated protein assemblies via hydroxamic acid functionalities Preparation of AIEgen-based near-infrared afterglow luminescence nanoprobes for tumor imaging and image-guided tumor resection 查看全部 Electroporation of fluorescent antisense molecules into Bodo saltans and live imaging Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way ASAPCRN iNDI Transcription Factor-NGN2 differentiation of human iPSC into cortical neurons Version 2 Obtain LB broth medium FLOUR-seq Cryptococcus neoformans DNA Extraction Method Passive avoidance (step-down test) U54 SCENT T/NK Immunosenescence Profiling Flow Cytometry Panel Workflow for wooden contact samples in use-wear experiments with bronze axe replicas (MAP-protocol_B) archaeology use-wear traceology bronze axes socketed axes contact materials contact samples experiment experimental archaeology bronze age beechwood controlled experiment Enriched Rat Housing 查看全部 Overview of Genotyping Technologies and Methods genetics genotyping GWAS methodology microarray NGS PCR Preparation of DNA and RNA Fragments Containing Guanine N2-Thioalkyl Tethers disulfide cross-links oligonucleotides Generating CRISPR-Cas9-Mediated Null Mutations and Screening Targeting Efficiency in Human Pluripotent Stem Cells clonal derivation CRISPR-Cas9 genome editing human pluripotent stem cells sgRNA efficiency STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries cis-regulatory element enhancer functional genomics gene expression gene regulation massively parallel reporter assay MPRA STARR-seq transcriptional regulation UMI-STARR-seq Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles EVs exosomes large-scale preparation MSCs Measuring Cytoskeletal Mechanical Fluctuations and Rheology with Active Micropost Arrays cytoskeleton magnetic actuation microposts microrheology Establishing an In Vitro 3D Spheroid Model to Study Medulloblastoma Drug Response and Tumor Dissemination 3D spheroids drug response medulloblastoma metastasis migration A Basic Guide to the Propagation and Manipulation of the Clubroot Pathogen, Plasmodiophora brassicae Cruciferous crops protist plant pathogen P. brassicae resting spores single spore isolate virulence Proteome-Derived Peptide Libraries for Deep Specificity Profiling of N-terminal Modification Reagents 2-pyridinecarboxaldehyde N terminomics protein N terminus proteome-derived peptide libraries subtiligase A OneStep Solution to Fix and Stain Cells for Correlative Live and Fixed Microscopy cell biology correlative microscopy cytoskeleton live imaging microscopy staining 查看全部