GENERATION OF RECOMBINANT αS AND PFF PROTOCOL Scott Vermilyea Published: 2024-03-21 DOI: 10.17504/protocols.io.81wgbxjx3lpk/v1 ASAPCRN AI 解读 浏览 1075 Abstract This is a collection of protocols including recombinant αS purification and αS PFF assembly. Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq Generation of heart-forming organoids from human pluripotent stem cells Design and fabrication of wearable electronic textiles using twisted fiber-based threads Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo Chromatin accessibility profiling by ATAC-seq Deoxyfluorination of phenols for chemoselective18F-labeling of peptides Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening 查看全部 Conditioned Media Concentration with Amicon Ultra Centrifugal Filters Conditioned Media Centrifugal Filters Proteomics Secretome SASP Collecting needle and branch samples for terpenoid and expression analysis mCherry-YIPF4 Immunoprecipitation V2 ASAPCRN Protocol for Safe, Affordable, and Reproducible Isolation of SARS-CoV-2 RNA from Wastewater RNA Wastewater Viral Concentration Variants RT-qPCR PEG TRIzol Wastewater-based epidemiology Nutrient solution for rice hydroponics culture rice hydroponics Yoshida's solution salinity Guidelines for microneutralization testing of human antibodies to West Nile virus Reproduction Analysis Short-Term Freezing of Anopheles stephensi (Mosquito) Larvae Combinatorial selective ER-phagy remodels the ER during neurogenesis ASAPCRN ER-phagy induced neurons axons whole cell proteomics differentiation live cell fluorescence microscopy Keima flux autophagy Wake Forest University Health Sciences Manual of Procedures Biospecimen Collection and Processing V4 查看全部 Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome Generation of Bispecific Antibodies by Functionalized Poly-ADP-Ribose Polymers antibody bispecific antibody chemical conjugation immunotherapy PARP1 poly-ADP-ribose post-translational modification High-Throughput Gene Replacement in Aspergillus fumigatus Aspergillus fumigatus fusion PCR genome-wide knockout library high-throughput gene editing Human Umbilical Cord Mesenchymal Stem Cell–Based in vitro Model for Neurotoxicity Testing cytotoxicity test mesenchymal stem cells neuron-like cells neuronal markers Culture of Rat Primary Cortical Cells for Microelectrode Array (MEA) Recordings to Screen for Acute and Developmental Neurotoxicity acute subchronic and chronic exposure microelectrode array (MEA) recordings neuronal activity analysis neurotoxicity screening rat primary cortical cultures Hi-C 3.0: Improved Protocol for Genome-Wide Chromosome Conformation Capture chromatin interactions chromosome conformation capture cross-linking Hi-C restriction enzyme Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Chloroplast Transformation in Arabidopsis Arabidopsis biolistic transformation chloroplast transformation plastid transformation transplastomic plant 查看全部
