Engineering SARS-CoV-2 using a reverse genetic system

Xuping Xie, Kumari G. Lokugamage, Xianwen Zhang, Michelle N. Vu, Antonio E. Muruato, Vineet D. Menachery, Pei-Yong Shi

Published: 2021-01-28 DOI: 10.1038/s41596-021-00491-8

SARS-CoV-2

reverse genetics

virus engineering

cDNA clone

COVID-19 research

AI 解读

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis

Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Tutorial: design and execution of CRISPR in vivo screens

Reproducible strategy for excisional skin-wound-healing studies in mice

CRISPR–Cas9-mediated chromosome engineering inArabidopsis thaliana

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

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Reverse genetic systems are a critical tool for studying viruses and identifying countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed an infectious complementary DNA (cDNA) clone for severe acute respiratory syndrome corona
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

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Menachery, V. D. et al. Combination attenuation offers strategy for live attenuated coronavirus vaccines. J. Virol. https://doi.org/10.1128/JVI.00710-18 (2018).
Menachery, V. D. et al. MERS-CoV accessory ORFs play key role for infection and pathogenesis. mBio https://doi.org/10.1128/mBio.00665-17 (2017).
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Engineering SARS-CoV-2 using a reverse genetic system

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