ELISA
Pranay Srivastava
Published: 2023-12-01 DOI: 10.17504/protocols.io.kqdg3xw67g25/v1
Abstract
This protocol is to determine the levels of IL-1β and TNF-α in plasma and brain (Srivastava et al. 2020).
Steps
1.
Blood samples from all the treatment groups were collected through cardiac puncture in 40 mM EDTA and plasma was collected by centrifugation.
2.
The plasma samples were immediately transferred to dry ice and then stored at -80°C till further analysis.
3.
A small fraction of ventral midbrain tissue homogenate prepared in 1x RIPA buffer (Cell Signaling, Cat#9806) was used for analysis of IL-1β and TNF-α using mouse ELISA kits (Biolegend Cat# 430904; 432604).
