DQ-BSA quantification
Narayana Yadavalli, Shawn M. Ferguson
Published: 2023-08-05 DOI: 10.17504/protocols.io.eq2ly7mzplx9/v1
Abstract
This protocol describes DQ-BSA quantification.
Attachments
Steps
DQ-BSA quantification
1.
Segment maximal projection images from z-stacks spanning complete cells by using the find maxima function.
2.
Threshold the duplicated images by default algorithm.
3.
Combine the segmented and thresholder images by AND function to create a mask.
4.
Obtain the mean gray values by applying analyze particle function to the mask and redirecting this whole analysis to the original images.
