DNA Extraction from Arabidopsis thaliana
lilyli
Abstract
This is a protocol for extract DNA from arabidopsis thialiana.
Steps
Add 400 mL DNA extraction solution into 1.5mL centrifuge tube.
Grind 3 small leaves or 1 large leaf with a grinding stick in the centrifuge tube with the extract.
Shake the oscillator for 0h 0m 5s, and place it at room temperature until other preparations are completed.
Centrifuge at 16 000 rpm for 2 minutes at room temperature.
Transfer 200 mL of centrifuged supernatant into a clean centrifuge tube.
Add 200 mL isopropanol, mix well and place at room temperature for 3 minutes.
Centrifuge at 16 000 rpm at room temperature for 5 minutes, remove the supernatant, wash the precipitate with 70% ethanol, and dry the precipitate at room temperature.
Dissolve DNA in 100 mL deionized water and store it in a refrigerator at – 20 ° C.
