CoBG-11 preparation
Arshshaikh
Published: 2021-10-18 DOI: 10.17504/protocols.io.by6hpzb6
Abstract
CoBG-11 is a coculture medium used to coculture cyanobacteria and E. coli by Zhang et al (2020). It is optimized for E. coli growth. Here are its components:
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150 mM NaCl,
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4 mM NH4Cl
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3 g/L 2-[[1,3-dihydroxy-2-(hydroxymethyl) propan-2-yl] amino] ethanesulfonic acid (TES)
The pH value is adjusted with NaOH to 8.3.
The protocol below describes the preparation of 100ml of coBG-11.
Steps
1. These calculations have been made for 100 mL coBG-11. For further information check the reference.
Zhang L, Chen L, Diao J, Song X, Shi M, Zhang W 2020 Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2. Biotechnology for biofuels https://doi.org/10.1186/s13068-020-01720-0
Measure 0.88g , 0.0214g, and 0.3g and add it to a 150mL flask.
Note
Citation
2.
Add 50mL to the flask and mix till contents dissolve. If salt persists, add 10mL more of BG-11 and mix well.
3.
Once contents dissolve, add BG-11 up to 95 mL.
4.
Adjust pH of the solution to 8.3 with NaOH.
5. Autoclave CoBG-11 before using.
Fill BG-11 up to 100 mL measure pH and adjust if required.
Note
