Bacterial genome engineering using CRISPR-associated transposases

Diego Rivera Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris H. Wang, Samuel H. Sternberg

Published: 2024-01-12 DOI: 10.1038/s41596-023-00927-3

genome engineering

CRISPR-associated transposases

bacterial genomes

DNA insertions

multiplexed edits

AI 解读

Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

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Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Combining surface-accessible Ag and Au colloidal nanomaterials with SERS for in situ analysis of molecule–metal interactions in complex solution environments

Generation of mouse–human chimeric embryos

Labeling and tracking of immune cells in ex vivo human skin

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing

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References

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Bacterial genome engineering using CRISPR-associated transposases

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