Akoya Biosciences PhenoCycler Fusion (formerly CODEX) User Guide

Andrew Houston

Published: 2024-01-12 DOI: 10.17504/protocols.io.x54v9p4j1g3e/v1
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Abstract

Link to PhenoCycler User Guide to allow for multi-plex spatial proteomic detection on fresh frozen and FFPE tissue sections

Steps

1.

Staining/slide prep protocol can be found here:

PhenoCycler-Fusion User Guide_2.1.0.pdf

FFPE tissue staining pgs. 45-56

Reporter plate preparation pgs. 70-75

2.

Imaging protocol can be found here:

PhenoImager Fusion User Guide_V1_1 Flow Cell_ 2.1.0.pdf

Flow cell assembly pgs. 29-39

Instrument set-up for PhenoCycler scan pgs. 57-71

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