Ai14 Genotyping
Ronald.zegarra
Published: 2023-03-12 DOI: 10.17504/protocols.io.81wgbywy1vpk/v1
Abstract
genotyping
Steps
1.
Thaw reagent on ice for0h 10m 0s
2.
PrepareSample:
3.
Pipette 19µL of Sample into 0.2 mL Tube
4.
Lightly Vortex DNA sample for 0h 0m 30s
5.
Add 1µL of DNA to each tube
6.
Select "Ai14" PCR protocol on the thermocycler
7.
Run 15 uL of PCR samples and a DNA ladder on a
at 120 V for 1h 0m 0s or until the dye is somewhat in the middle of the gel.
