Ai14 Genotyping
Ronald.zegarra
Published: 2023-03-12 DOI: 10.17504/protocols.io.81wgbywy1vpk/v1
Abstract
genotyping
Steps
1.
Thaw reagent on ice for0h 10m 0s
2.
PrepareSample
:
3.
Pipette 19µL
of Sample
into 0.2 mL Tube
4.
Lightly Vortex DNA sample for 0h 0m 30s
5.
Add 1µL
of DNA to each tube
6.
Select "Ai14" PCR protocol on the thermocycler
7.
Run 15 uL of PCR samples and a DNA ladder on a
at 120 V for 1h 0m 0s
or until the dye is somewhat in the middle of the gel.