10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex -- University of Minnesota TMCs (CG000527 Rev E)
IOx Genomics, Laura Niedernhofer, David A Bernlohr
Published: 2024-03-23 DOI: 10.17504/protocols.io.bp2l6x81klqe/v2
Abstract
10x Genomics Chromium Single Cell Expression flex protocol for library construction.
Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Steps
Preparation
1.
Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
Library Preparation
2.
Sequence with the read format 29,10,10,89
Note
FASTQ Generation
3.
BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq version 2.20.0
