FastDNA SPIN Kit for Soil

The FastDNATM SPIN Kit for Soil quickly and efficiently isolates PCR-ready genomic DNA directly from soil samples in less than 30 minutes. Designed for use with the FastPrep® instruments from MP Biomedicals, plant and animal tissues, bacteria, algae, fungal spores and other members of a soil population are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles. FastPrep instruments provide a quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods using enzymatic digestion, sonication, blending, douncing and vortexing.

Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all soil organisms, including historically difficult sources, such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi. Homogenization in a FastPrep instrument with Lysing Matrix E takes place in the presence of MT Buffer and Sodium Phosphate Buffer, reagents carefully developed to protect and solubilize nucleic acids and proteins upon cell lysis. These reagents work synergistically to allow extraction of genomic DNA with minimal RNA contamination.

Following lysis, samples are centrifuged to pellet soil, cell debris and lysing matrix. DNA is purified from the supernatant with the Binding Matrix FastDNA procedure using SPIN filters. Eluted DNA is ready for PCR, restriction digest, electrophoresis and any other desired application.