实验方法库
覆盖上万篇生物顶级期刊，专业引领，成就科研速度！
筛选
根据 “2021 年份” 筛选结果如下：
Generating Mutant Renal Cell Lines Using CRISPR Technologies
Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David. A. Long, Dagan Jenkins
Immunoprecipitation Washes and RNA Dephosphorylation
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer
Matthew B. Robers, James D. Vasta, Cesear R. Corona, Rachel Friedman Ohana, Robin Hurst, Manisha A. Jhala, Kenneth M. Comess, Keith V. Wood
3.6 Coculturing with Astrocytes
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
3.3 Lentivirus Production and Transduction
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
3' RNA Adapter Ligation to Input RNA
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
3.4 Genome Editing with CRISPR/Cas9
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
3.5 Doxycycline-Induced Differentiation
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
Microinjection Techniques in Fly Embryos to Study the Function and Dynamics of SMC Complexes
SMC complexes
Cohesin
Condensin
Drosophila melanogaster
Syncytial embryo
Microinjections
TEV protease
Fluorescence recovery after photobleaching (FRAP)
FRAP
Catarina Carmo, Margarida Araújo, Raquel Oliveira
3.2 Nucleofection of iPSCs
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
