实验方法库
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筛选
根据 “2021 年份” 筛选结果如下:
Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells
Cell-free protein synthesis
CHO cells
Continuous exchange cell-free systems
Difficult-to-express proteins
In vitro protein production
Internal ribosomal entry sites
Linear expression templates
Lena Thoring, Stefan Kubick
2021-09-03
Ligate 3′ RNA Adapter (On-Bead)
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
2021-09-03
Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria
Fluorescent-based redox proteomics
Thiol trapping
Mass spectrometry
Reversible thiol oxidations
Protein S-thiolations
Martina Rossius, Falko Hochgräfe, Haike Antelmann
2021-09-03
3.2 Production of a Cleared Cell Lysate
Rho GTPase
Rab GTPase
Cell signaling
Cytoskeleton
Hantavirus
Flow cytometry
Integrin activation
Sepsis
Multiplex
Protease-activated receptors
PARs
Thrombin
Argatroban
Bead functionalization
Glutathione-S-transferase
GST
GTPase effector beads
Rap1
RhoA
Rac1
Rab7
Fluorescence calibration beads
Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
2021-09-03
3.3 Molecular Assembly of GST Effector Proteins on Glutathione Beads
Rho GTPase
Rab GTPase
Cell signaling
Cytoskeleton
Hantavirus
Flow cytometry
Integrin activation
Sepsis
Multiplex
Protease-activated receptors
PARs
Thrombin
Argatroban
Bead functionalization
Glutathione-S-transferase
GST
GTPase effector beads
Rap1
RhoA
Rac1
Rab7
Fluorescence calibration beads
Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
2021-09-03
Elution, SDS-PAGE, and RNA Purification
RNA degradation
Protein–RNA interaction
RNA-binding sites
UV cross-linking
Yeast
Exosome
RNA processing
Clémentine Delan-Forino, David Tollervey
2021-09-03
3.4 Assay Runtime
Rho GTPase
Rab GTPase
Cell signaling
Cytoskeleton
Hantavirus
Flow cytometry
Integrin activation
Sepsis
Multiplex
Protease-activated receptors
PARs
Thrombin
Argatroban
Bead functionalization
Glutathione-S-transferase
GST
GTPase effector beads
Rap1
RhoA
Rac1
Rab7
Fluorescence calibration beads
Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
2021-09-03
Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases
Rho GTPase
Rab GTPase
Cell signaling
Cytoskeleton
Hantavirus
Flow cytometry
Integrin activation
Sepsis
Multiplex
Protease-activated receptors
PARs
Thrombin
Argatroban
Bead functionalization
Glutathione-S-transferase
GST
GTPase effector beads
Rap1
RhoA
Rac1
Rab7
Fluorescence calibration beads
Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
2021-09-03
3.1 Synthesis of Glutathione Beads
Rho GTPase
Rab GTPase
Cell signaling
Cytoskeleton
Hantavirus
Flow cytometry
Integrin activation
Sepsis
Multiplex
Protease-activated receptors
PARs
Thrombin
Argatroban
Bead functionalization
Glutathione-S-transferase
GST
GTPase effector beads
Rap1
RhoA
Rac1
Rab7
Fluorescence calibration beads
Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
2021-09-03