实验方法库
覆盖上万篇生物顶级期刊，专业引领，成就科研速度！
筛选
根据 “2021 年份” 筛选结果如下：
Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
UV Denaturation of Proteins Monitored by Circular Dichroism in the Far-UV Region (180–260 nm)
Circular dichroism
Ligand binding
Titration
Binding constant
UV denaturation
Protein stability
Data processing
Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
3.1 Human iPSC Culture
Human induced pluripotent stem cells
Nucleofection
PiggyBac transposon
Lentiviral transduction
CRISPR/Cas9
Transcription factor-mediated neuronal differentiation
Astrocyte coculture
Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS)
Lung
Cell type-specific in vivo pharmacology
Oligonucleotides
Fluorescence activated cell sorting
Magnetic cell sorting
RNAi
siRNA
Antisense
MicroRNA
Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos
Enzyme–Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism
Circular dichroism
Ligand binding
Titration
Binding constant
UV denaturation
Protein stability
Data processing
Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
3.1 Preparation of Monocellular Lung Suspension from a Mouse Lung Lobe
Lung
Cell type-specific in vivo pharmacology
Oligonucleotides
Fluorescence activated cell sorting
Magnetic cell sorting
RNAi
siRNA
Antisense
MicroRNA
Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos
Standard CD Measurements
Circular dichroism
Ligand binding
Titration
Binding constant
UV denaturation
Protein stability
Data processing
Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
Dephosphorylation of Input RNA
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
Western Blotting and RNA Isolation from Membrane
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
3.2 Isolation of Specific Cell Types from Digested Lung Tissue
Lung
Cell type-specific in vivo pharmacology
Oligonucleotides
Fluorescence activated cell sorting
Magnetic cell sorting
RNAi
siRNA
Antisense
MicroRNA
Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos
