实验方法库

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RNA-Seq

Transforming

Data analysis

CRISPR

Genetic

Preparation and culture of mouse intestinal organoids

ASAPCRN

Protocol for vagus nerve stimulation in awake pigs (subject-4)

OT-2 Counter-Selection

Measuring Protein-Protein Interactions and Quantifying Their Dissociation Constants with Mass Photometry

antibodies

binding affinity

complex formation

dissociation constant

KD

protein A

protein-protein interactions

Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence.

ASAPCRN

GPNMB

Western Blotting

Immunofluorescence

HEK293

Real time-quaking induced conversion assay (RT-QUIC)

ASAPCRN

Preparation of mouse tissue homogenates for RT-QuIC assay

ASAPCRN

alpha-synuclein

tissue homogenation

rt-quic

Isolation and co-culture of nodose ganglion neurons

ASAPCRN

Differentiation of Astrocytes from Human iPSC-derived NPCs

Astrocyte

hiPSC

Neural progenitor cell

Vagotomy and Tamoxifen treatment

Vagotomy

Tamoxifen treatment

Vil-CreERT2 mice

ASAPCRN

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