实验方法库
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筛选
根据 “Protocols IO 数据源” 筛选结果如下:
Western Blotting and RNA Isolation from Membrane
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
Dephosphorylation of Input RNA
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
3' RNA Adapter Ligation to Input RNA
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
Generation of Library for Sequencing
RNA degradation
Protein–RNA interaction
RNA-binding sites
UV cross-linking
Yeast
Exosome
RNA processing
Clémentine Delan-Forino, David Tollervey
Cell Lysis and RNA Fragmentation
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
Cell Lysis, Two-Step Purification, and RNase Digestion
RNA degradation
Protein–RNA interaction
RNA-binding sites
UV cross-linking
Yeast
Exosome
RNA processing
Clémentine Delan-Forino, David Tollervey
Enzyme–Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism
Circular dichroism
Ligand binding
Titration
Binding constant
UV denaturation
Protein stability
Data processing
Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi